Materials+and+Methods

= = __** Provide a comprehensive list of all materials, equipment and chemicals in a table. Quantity Required || Description of Equipment (Brand: Shultz fertilizer, composition: plastic, glass, etc., size: 500 mL pot, concentration, amount, 10 g of fertilizer, etc || Source of Equipment || 1 || 200 mL Pyrex graduated cylinder || CDS Lab, Escazu, Costa Rica ||
 * Materials: How To Also** **__Download link at end of doc

Sample List **MATERIALS** 40 || 20 cm Plastic Pots || EPA, Guachipilin, Costa Rica || 4 || Plastic trays to hold 10 pots each || CDS Lab, Escazu, Costa Rica || 40 || Chili Plants //Capsicum annum// || Vivero El Encantado, Escazu, Costa Rica || 1 box || 1000 g Concentrated Miracle Grow Vegetable Fertilizer || EPA, Guachipilin, Costa Rica || 1 box || 1000 g Shultz Vegetable Fertilizer || EPA, Guachipilin, Costa Rica || 1 bag || 1 kg cow manure || Pasture on uncles finca, San Antonio de Escazu, Costa Rica || 1 bag || 5 kg gardening soil || Pasture on uncles finca, San Antonio de Escazu, Costa Rica || 4 liters || Tap Water || faucet ||



Guidelines || Examples || Use numbered steps
 * Methods: How To**

Organize steps into small individual directions in the order you would do them in the experiment || 1. Record date, site condition where plants will be placed. 2. Place each individual chili plants into individual 20 cm plastic pots, for a total of 40 plants. 3. Using the same soil source, be sure that soil and plant are approximately 2 cm from the top lip of the pots. 4. Place 10 potted plants in 1 tray. 5. Repeat step 4 for each tray so there are 10 plants in each tray || Avoid using pronouns || I, you, she, he || Use commands || Record date || Explain how you selected organisms or test subjects || Choose 40 plants of the same age and size from the same source

Use a table of random digits to select 10 subjects from your list of volunteers. || Tell reader to measure and record data when it is appropriate. Explain what to record and how to measure. || 1. Prepare experimental soil by mixing in 30 g of fertilizer into soil. 2. Using the same soil source, be sure that soil and plant are approximately 2 cm from the top lip of the pots. || Refer only to metric measurements || g, m, mm, cm, sec, mL, etc. || Eliminate extraneous steps || Get out equipment Go to site Take out paper and pencil || Sketch steps where appropriate and refer to them || 1. Using the same soil source, be sure that soil and plant are approximately 2 cm from the top lip of the pots. (see figure 1) || Explain how to keep constants the same || Place seedlings trays side by side in location so that all plants receive the same amount of sun. || All equipment in materials section will appear in your methods || || Tell experimenter when to repeat steps || Repeat steps 7-9 for second, third, and fourth trays. || The methods section provides a comprehensive list of exact steps for replicating your experiment and for controlling the outcome. It must detail exactly how you will implement constants, create the control groups and experimental groups. It must explain how you will measure the IV and DV. Sample Methods 1.  Use 4 small pots the same size. Make sure they have drainage holes at the bottom. Styrofoam or waxed paper cups are OK. 2. Fill with soil. Up to 2 cm from rim of pot. (see figure 1) 3. Moisten the soil and let excess water drain. 4. Sprinkle 8-10 ordinary radish seeds on the soil. 5. Now sprinkle about 1/4 inch of soil over the seeds and water again, but very lightly. 6. Water very lightly every day with ordinary tap water. 7. Record amount of water, and be sure each specimen receives the same amount. 8. <span style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-fareast-font-family: "Times New Roman";">After the radishes sprout from the soil, place all the pots together, preferably outdoors in a sunny location. <span style="font-family: Arial,sans-serif; font-size: 12pt;">9. <span style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-fareast-font-family: "Times New Roman";">Be sure that all plants receive the same amount of light <span style="font-family: Arial,sans-serif; font-size: 12pt;">10. <span style="font-family: "Arial","sans-serif"; font-size: 12.0pt; mso-fareast-font-family: "Times New Roman";">Label each pot with a name or number. Water lightly every day, each with different water: <span style="font-family: Arial,sans-serif; font-size: 14pt;">a. <span style="font-family: "Arial","sans-serif"; font-size: 14.0pt; mso-fareast-font-family: "Times New Roman";">water from your kitchen tap <span style="font-family: Arial,sans-serif; font-size: 14pt;">b. <span style="font-family: "Arial","sans-serif"; font-size: 14.0pt; mso-fareast-font-family: "Times New Roman";">rain water (from roof collected in a bucket) <span style="font-family: Arial,sans-serif; font-size: 14pt;">c. <span style="font-family: "Arial","sans-serif"; font-size: 14.0pt; mso-fareast-font-family: "Times New Roman";">spring water (from the store) <span style="font-family: Arial,sans-serif; font-size: 14pt;">d. <span style="font-family: "Arial","sans-serif"; font-size: 14.0pt; mso-fareast-font-family: "Times New Roman";">pond, creek, lake, canal or river water <span style="font-family: arial,helvetica,sans-serif; font-size: 18px; line-height: 27px;"> <span style="font-family: arial,helvetica,sans-serif; font-size: 18px; line-height: 27px;"> =**ExD and Materials and Methods more details.**=